The effects of zinc sulphate and azelaic acid on 5 alpha-reductase activity in human skin were studied using an in vitro assay with 1,2[3H]-testosterone as substrate. When added at concentrations of 3 or 9 mmol/l, zinc was a potent inhibitor of 5 alpha-reductase activity. At high concentrations, zinc could completely inhibit the enzyme activity. Azelaic acid was also a potent inhibitor of 5 alpha-reductase; inhibition was detectable at concentrations as low as mmol/l and was complete at 3 mmol/l. An additive effect of the two inhibitors was observed. Vitamin B6 potentiated the inhibitory effect of zinc, but not of azelaic acid, suggesting that two different mechanisms are involved. When the three substances were added together at very low concentrations which had been shown to be ineffective alone, 90% inhibition of 5 alpha-reductase activity was obtained. If this inhibition is confirmed in vivo, zinc sulphate combined with azelaic acid could be an effective agent in the treatment of androgen related pathology of human skin.
The price can range wildly from around £12 for a single 60ml bottle of regular strength Regaine to £ for a six month supply of extra strength Regaine. In the end the cost depends on where you look (search around as you will generally be able to find it at discount prices on various websites) and how much you are willing to buy at any one time. Generally it is better to buy it in bulk (as with everything else) as this makes it cheaper, and, because it is something you will have to use indefinitely, then it is worth shopping around and getting larger orders to bring down the cost.
Provillus is only available on its official website, because manufacturers are concerned with their product’s authenticity. Therefore, in order to make sure that the Provillus clients purchase is not a fake, they encourage prospective customers to purchase from the product’s official website. There are also plenty of perks when it comes to this method of purchase, namely the 90-day money-back guarantee and great deals when a bigger supply is bought. As reviews show, the best option is to order the six-month supply, because budget wise it means that you pay a little over a dollar per day.